To bolster our understanding of P. vivax–An. albimanus midgut interactions, we developed an integrated bioinformatic-hybrid RNA-Seq-LC-MS/MS approach involving An. albimanus transcriptome (15,764 contigs) and luminal midgut subproteome (9,445 proteins) assembly, which, when used with our custom Diptera protein database (685,078 sequences), facilitated a comparative proteomic analysis of the midgut brush borders of two important malaria vectors, An. gambiae and An. albimanus.
Malaria vectors in sub-Saharan Africa have proven themselves very difficult adversaries in the global struggle against malaria.
During the survey period (May--June) in this forested area of Cote d'Ivoire, An. gambiae s. s. found were exclusively of the M form and were apparently selected for pyrethroid resistance through agricultural and household usage of insecticides.
The larvae of An. gambiae s.s laboratory colony and An. gambiae s.l of wild populations were assayed and the larval mortality was observed at 12, 24 and 48 h after exposure period with the concentrations of 3.125, 6.25, 12.5, 25, 50 and 100 ppm.
Malaria transmission was monitored in two villages in the Sahel zone of Niger over 4 years.
The impact of insecticide resistance on insect-borne disease programs is difficult to quantify.
This guest editorial was submitted to MalariaWorld by Prof. Maureen Coetzee, Nora Besansky, Alessandra della Torre and Rick Wilkerson.
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