The world's scientific and social network for malaria professionals
Subscribe to free Newsletter | 10858 malaria professionals are enjoying the free benefits of MalariaWorld today

RDT

NOT Open Access | Ultrasensitive diagnostics for low density asymptomatic Plasmodium falciparum infections in low transmission settings

November 7, 2020 - 13:00 -- NOT Open Access
Author(s): 
Zainabadi K
Reference: 
J Clin Microbiol. 2020 Nov 4:JCM.01508-20

The emergence of multidrug resistant Plasmodium falciparum malaria in Southeast Asia has accelerated regional malaria elimination efforts. Most malaria in this and other low transmission settings exists in asymptomatic individuals, which conventional diagnostic tests lack the sensitivity to detect. This has led to the development of new ultrasensitive diagnostics that are capable of detecting these low parasitemic infections.

Subsidise the test, the treatment or both? Results of an individually randomised controlled trial of the management of suspected malaria fevers in the retail sector in western Kenya

November 7, 2020 - 12:57 -- Open Access
Author(s): 
Laktabai J, Saran I, Zhou Y, Simmons RA, Turner EL, Visser T, O'Meara W
Reference: 
BMJ Glob Health. 2020 Nov;5(11):e003378

In many malaria-endemic countries, the private retail sector is a major source of antimalarial drugs. However, the rarity of malaria diagnostic testing in the retail sector leads to overuse of the first-line class of antimalarial drugs known as artemisinin-combination therapies (ACTs). The goal of this study was to identify the combination of malaria rapid diagnostic test (RDT) and ACT subsidies that maximises the proportion of clients seeking care in a retail outlet that choose to purchase an RDT (RDT uptake) and use ACTs appropriately.

Community-based surveys for Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions in selected regions of mainland Tanzania

November 4, 2020 - 15:14 -- Open Access
Author(s): 
Catherine Bakari, Sophie Jones, Deus S. Ishengoma, et al.
Reference: 
Malaria Journal 2020 19:391, 4 November 2020

Histidine-rich protein 2 (HRP2)-based malaria rapid diagnostic tests (RDTs) are effective and widely used for the detection of wild-type Plasmodium falciparum infections. Although recent studies have reported false negative HRP2 RDT results due to pfhrp2 and pfhrp3 gene deletions in different countries, there is a paucity of data on the deletions of these genes in Tanzania.

Genetic variations in histidine-rich protein 2 and histidine-rich protein 3 of Myanmar Plasmodium falciparum isolates

November 4, 2020 - 10:02 -- Open Access
Author(s): 
Hương Giang Lê, Jung-Mi Kang, Jinyoung Lee, Won Gi Yoo, Moe Kyaw Myint, Khin Lin, Tong-Soo Kim and Byoung-Kuk Na
Reference: 
Malaria Journal 2020 19:388, 2 November 2020

Malaria rapid diagnostic tests (RDTs) are precious tools to diagnose malaria. Most RDTs used currently are based on the detection of Plasmodium falciparum histidine-rich protein 2 (PfHRP2) in a patient’s blood. However, concern has been raised in recent years that deletion of pfhrp2 in the parasite could affect the accuracy of PfHRP2-based RDTs. In addition, genetic variation in pfhrp2 might influence the accuracy and sensitivity of RDTs. In this study, the genetic variation in pfhrp2 and pfhrp3 in Myanmar P. falciparum isolates was analysed.

NOT Open Access | HRP2/3 Mutation in Recrudescent Plasmodium falciparum Malaria Case Acquired in Ethiopia

October 21, 2020 - 08:17 -- NOT Open Access
Author(s): 
Gefen-Halevi S, Belinson V, Manor U, Gazit Z, Smollan G, Belausov N, Biber A, Schwartz E, Grossman T, Leshem E, Amit S
Reference: 
J Travel Med. 2020 Oct 15:taaa196

A 65-year-old Israeli working in Welkait, Ethiopia, not using malaria prophylaxis, developed fever. Malaria RDT was consistent with non-falciparum malaria (plasmodium LDH+/HRP-) but microscopy showed typical P. falciparum.

Cost-effectiveness analysis of malaria rapid diagnostic test in the elimination setting

October 1, 2020 - 15:36 -- Open Access
Author(s): 
Du YQ, Ling XX, Jin JJ, Zhou HY, Zhu S, Zhu GD, Wang W, Cao J, Huang JY
Reference: 
Infect Dis Poverty. 2020 Sep 29;9(1):135

As more and more countries approaching the goal of malaria elimination, malaria rapid diagnostic tests (RDT) was recomendated to be a diagnostic strategy to achieve and maintain the statute of malaria free, as it’s less requirments on equipment and experitise than microscopic examination. But there are very few economic evaluations to confirm whether RDT was cost-effective in the setting of malaria elimination. This research aimed to offer evidence for helping decision making on malaria diagnosis strategy.

Evaluating the potential of using urine and saliva specimens for malaria diagnosis in suspected patients in Ghana

October 1, 2020 - 15:32 -- Open Access
Author(s): 
Enoch Aninagyei, Joseph Abraham, Paul Atiiga, Shadrach Duodu Antwi, Stephen Bamfo and Desmond Omane Acheampong
Reference: 
Malaria Journal 2020 19:349, 29 September 2020

This study aimed at detecting PfHRP2 and pLDH malaria antigens in urine and salivary specimens of suspected malaria patients using RDT kits, and identifying factors influencing the detection of these antigens.

Contribution of P. falciparum parasites with Pfhrp 2 gene deletions to false negative PfHRP 2 based malaria RDT results in Ghana: A nationwide study of symptomatic malaria patients

September 8, 2020 - 12:32 -- Open Access
Author(s): 
Amoah LE, Abuaku B, Bukari AH, Dickson D, Amoako EO, Asumah G, Asamoah A, Preprah NY, Malm KL
Reference: 
PLoS One. 2020 Sep 4;15(9):e0238749

False-negative malaria rapid diagnostic test (RDT) results amongst symptomatic malaria patients are detrimental as they could lead to ineffective malaria case management. This study determined the nationwide contribution of parasites with Pfhrp2 and Pfhrp 3 gene deletions to false negative malaria RDT results in Ghana.

Insecticide resistance in indoor and outdoor-resting Anopheles gambiae in Northern Ghana

September 1, 2020 - 10:24 -- Open Access
Author(s): 
Majidah Hamid-Adiamoh, Alfred Amambua-Ngwa, Davis Nwakanma, Umberto D’Alessandro, Gordon A. Awandare and Yaw A. Afrane
Reference: 
Malaria Journal 2020 19:314, 31 August 2020

Selection pressure from continued exposure to insecticides drives development of insecticide resistance and changes in resting behaviour of malaria vectors. There is need to understand how resistance drives changes in resting behaviour within vector species. The association between insecticide resistance and resting behaviour of Anopheles gambiae sensu lato (s.l.) in Northern Ghana was examined.

Active case detection of malaria in pregnancy using loop-mediated amplification (LAMP): a pilot outcomes study in South West Ethiopia

September 1, 2020 - 09:45 -- Open Access
Author(s): 
Guluma Tadesse, Claire Kamaliddin, Cody Doolan, Ranmalee Amarasekara, Ruth Legese, Abu Naser Mohon, James Cheaveau, Delenasaw Yewhalaw and Dylan R. Pillai
Reference: 
Malaria Journal 2020 19:305, 27 August 2020

125 million women are pregnant each year in malaria endemic areas and are, therefore, at risk of Malaria in Pregnancy (MiP). MiP is the direct consequence of Plasmodium infection during pregnancy. The sequestration of Plasmodium falciparum parasites in the placenta adversely affects fetal development and impacts newborn birth weight. Importantly, women presenting with MiP commonly develop anaemia. In Ethiopia, the Ministry of Health recommends screening symptomatic women only at antenatal care visits with no formal intermittent preventive therapy. Since MiP can display low-level parasitaemia, current tests which include microscopy and RDT are challenged to detect these cases. Loop mediated isothermal Amplification (LAMP) technology is a highly sensitive technique for DNA detection and is field compatible. This study aims to evaluate the impact of active malaria case detection during pregnancy using LAMP technology in terms of birth outcomes.

Pages

Subscribe to RSS - RDT