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RDTs

Summary of discordant results between rapid diagnosis tests, microscopy, and polymerase chain reaction for detecting Plasmodium mixed infection: a systematic review and meta-analysis

July 30, 2020 - 14:10 -- Open Access
Author(s): 
Kotepui M, Kotepui KU, De Jesus Milanez G, Masangkay FR
Reference: 
Sci Rep. 2020 Jul 29; 10(1):12765

Malaria rapid diagnostic tests (RDTs) are widely used to detect malaria parasites among patients who suspected malaria infections in malaria-endemic areas where microscopy is unavailable. Nevertheless, little is known about the performance of RDTs in detecting Plasmodium mixed infections. The present study aimed to evaluate the discordant results between RDTs and microscopy/polymerase chain reaction (PCR) in detecting Plasmodium mixed infections.

One-step PCR: A novel protocol for determination of pfhrp2 deletion status in Plasmodium falciparum

July 27, 2020 - 12:21 -- Open Access
Author(s): 
Jones S, Subramaniam G, Plucinski MM, Patel D, Padilla J, Aidoo M, Talundzic E
Reference: 
PLoS ONE 15(7): e0236369

Histidine-rich protein 2 (HRP2) detecting rapid diagnostic tests (RDTs) have played an important role in enabling prompt malaria diagnosis in remote locations. However, emergence of pfhrp2 deleted parasites is threatening the efficacy of RDTs, and the World Health Organization (WHO) has highlighted surveillance of these deletions as a priority. Nested PCR is used to confirm pfhrp2 deletion but is costly and laborious.

Plasmodium falciparum histidine-rich protein 2 diversity in Ghana

July 20, 2020 - 15:10 -- Open Access
Author(s): 
Otchere Addai-Mensah, Bismarck Dinko, Alexander Yaw Debrahq, et al.
Reference: 
Malaria Journal 2020 19:256, 16 July 2020

In the absence of microscopy, Plasmodium falciparum histidine-rich proteins 2 (PfHRP2)-based rapid diagnostic tests (RDTs) are recommended for the diagnosis of falciparum malaria, particularly in endemic regions. However, genetic variability of the pfhrp2 gene threatens the usefulness of the test due to its impact on RDT sensitivity. This study aimed to investigate the diversity of pfhrp2 in malaria cases among children in Ghana.

Systematic review and meta-analysis of diagnostic accuracy of Loop-mediated isothermal amplification (LAMP) methods compared to microscopy, PCR, and rapid diagnostic tests, for malaria diagnosis

July 14, 2020 - 15:50 -- Open Access
Author(s): 
Picot S, Cucherat M, Bienvenu AL
Reference: 
Int J Infect Dis. 2020 Jul 10:S1201-9712(20)30551-8

Diagnosis is a challenging issue in the way to malaria elimination. LAMP could be an alternative to conventional methods. Then, it is of interest to evaluate the diagnostic accuracy of LAMP for malaria compared to microscopy, PCR, and RDTs.

Evaluating the implementation of rapid diagnostic tests in a malaria elimination setting

July 13, 2020 - 16:14 -- Open Access
Author(s): 
Liang D, Jin JJ, Wang WM, Cao YY, Zhu GD, Zhou HY, Cao J, Huang JY
Reference: 
Infect Dis Poverty. 2020 Jul 8;9(1):84

It was recommended that malaria rapid diagnostic tests (RDTs) should be available in all epidemiological situations. But evidence was limited on the implementation of RDTs and its effectiveness in malaria elimination settings. This study examined the implementation of RDTs and how it affected the diagnosis of imported malaria patients in Jiangsu Province, China.

Defining symptoms of malaria in India in an era of asymptomatic infections

July 7, 2020 - 13:58 -- Open Access
Author(s): 
Anna Maria van Eijk, Asad S. Mannan, Steven A. Sullivan and Jane M. Carlton
Reference: 
Malaria Journal 2020 19:237, 6 July 2020

Malaria is a major public health problem in India. Data from surveys totaling 3031 participants at three sites revealed a high proportion of asymptomatic infections, complicating diagnosis. The aim of this study was to identify differences in complaints and symptoms between sites, and factors associated with asymptomatic Plasmodium infections.

Molecular detection and quantification of Plasmodium falciparum gametocytes carriage in used RDTs in malaria elimination settings in northern Senegal

March 31, 2020 - 15:17 -- Open Access
Author(s): 
Kiswendsida Thierry Guiguemde, Yakou Dieye, Babacar Faye, et al.
Reference: 
Malaria Journal 2020 19:123, 30 March 2020

Malaria surveillance requires powerful tools and strategies to achieve malaria elimination. Rapid diagnostic tests for malaria (RDTs) are easily deployed on a large scale and are helpful sources of parasite DNA. The application of sensitive molecular techniques to these RDTs is a modern tool for improving malaria case detection and drug resistance surveillance. Several studies have made it possible to extract the DNA of Plasmodium falciparum from RDTs. The knowledge of gametocyte carriage in the population is important to better assess the level of parasite transmission in elimination settings. The aim of this study was to detect P. falciparum gametocytes from used RDTs by quantitative PCR for molecular monitoring of malaria transmission.

Do you see patients with cerebral malaria? ...Or know someone who does?

March 21, 2014 - 11:16 -- Lakshmi Swamy

If you care for patients with cerebral malaria or know someone who does, then we would appreciate your help in distributing this short survey.
Please use the following link to complete the survey: Cerebral Malaria Diagnosis Survey

I am an ophthalmologist with a background in malaria biology, and I am leading a survey study to understand how clinicians diagnose cerebral malaria.

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