The world's scientific and social network for malaria professionals
Subscribe to free Newsletter | 10623 malaria professionals are enjoying the free benefits of MalariaWorld today

RDTs

Demonstration of indigenous malaria elimination through Track-Test-Treat-Track (T4) strategy in a Malaria Elimination Demonstration Project in Mandla, Madhya Pradesh

September 17, 2020 - 13:52 -- Open Access
Author(s): 
Praveen K. Bharti, Harsh Rajvanshi, Altaf A. Lal, et al.
Reference: 
Malaria Journal 2020 19:339, 17 September 2020

Many malaria endemic countries are heading towards malaria elimination through the use of case management and vector control strategies, which employ surveillance, improving access to early diagnosis, prompt treatment., and integrated vector control measures. There is a consensus that elimination of malaria is feasible when rapid detection and prompt treatment is combined with mosquito-human contact interruption in an efficient and sustainable manner at community levels. This paper describes results of an integrated case management and vector control strategy for reducing malaria cases in 1233 villages over 3 years in district Mandla, Madhya Pradesh, India.

Evaluation of the colorimetric malachite green loop-mediated isothermal amplification (MG-LAMP) assay for the detection of malaria species at two different health facilities in a malaria endemic area of western Kenya

September 10, 2020 - 08:55 -- Open Access
Author(s): 
James Gachugia, Winnie Chebore, Kephas Otieno, Caroline Wangari Ngugi, Adano Godana and Simon Kariuki
Reference: 
Malaria Journal 2020 19:329, 9 September 2020

Prompt diagnosis and effective malaria treatment is a key strategy in malaria control. However, the recommended diagnostic methods, microscopy and rapid diagnostic tests (RDTs), are not supported by robust quality assurance systems in endemic areas. This study compared the performance of routine RDTs and smear microscopy with a simple molecular-based colorimetric loop-mediated isothermal amplification (LAMP) at two different levels of the health care system in a malaria-endemic area of western Kenya.

Molecular surveillance reveals the presence of pfhrp2 and pfhrp3 gene deletions in Plasmodium falciparum parasite populations in Uganda, 2017–2019

August 27, 2020 - 08:09 -- Open Access
Author(s): 
Agaba B. Bosco, Karen Anderson, Karryn Gresty, et al
Reference: 
Malaria Journal 2020 19:300, 26 August 2020

Histidine-rich protein-2 (HRP2)-based rapid diagnostic tests (RDTs) are the only RDTs recommended for malaria diagnosis in Uganda. However, the emergence of Plasmodium falciparum histidine rich protein 2 and 3 (pfhrp2 and pfhrp3) gene deletions threatens their usefulness as malaria diagnostic and surveillance tools. The pfhrp2 and pfhrp3 gene deletions surveillance was conducted in P. falciparum parasite populations in Uganda.

NOT Open Access | Prevalence of Asymptomatic Malaria Parasitemia in Odisha, India: A Challenge to Malaria Elimination

August 17, 2020 - 13:08 -- NOT Open Access
Author(s): 
Kumari P, Sinha S, Gahtori R, Yadav CP, Pradhan MM, Rahi M, Pande V, Anvikar AR
Reference: 
Am J Trop Med Hyg. 2020 Aug 10

The prevalence of malaria in India is decreasing, but it remains a major concern for public health administration. The role of submicroscopic malaria and asymptomatic malaria parasitemia and their persistence is being explored. A cross-sectional survey was conducted in the Kandhamal district of Odisha (India) during May-June 2017. Blood samples were collected from 1897 individuals for screening of asymptomatic parasitemia. Samples were screened using rapid diagnostic tests (RDTs) and examined microscopically for Plasmodium species.

Emergence of Undetectable Malaria Parasites: A Threat under the Radar amid the COVID-19 Pandemic

August 13, 2020 - 11:05 -- Open Access
Author(s): 
Beshir KB, Grignard L, Hajissa K, Mohammed A, Nurhussein AM, Ishengoma DS, Lubis IND, Drakeley CJ, Sutherland CJ
Reference: 
Am J Trop Med Hyg. 2020 Aug;103(2):558-560

Rapid diagnostic tests (RDTs) play a critical role in malaria diagnosis and control. The emergence of Plasmodium falciparum parasites that can evade detection by RDTs threatens control and elimination efforts. These parasites lack or have altered genes encoding histidine-rich proteins (HRPs) 2 and 3, the antigens recognized by HRP2-based RDTs.

School-based sero-surveys to assess the validity of using routine health facility data to target malaria interventions in the Central Highlands of Madagascar

August 10, 2020 - 15:07 -- Open Access
Author(s): 
Steinhardt L, Ravaoarisoa E, Vigan-Womas I, et al.
Reference: 
J Infect Dis. 2020 Aug 6:jiaa476

In low-malaria-transmission areas of Madagascar, annual parasite incidence (API) from routine data has been used to target indoor residual spraying at sub-district commune levels. To assess validity of this approach, we conducted school-based serological surveys and health facility (HF) data quality assessments in seven districts to compare API to “gold-standard” commune-level serological measures.

Summary of discordant results between rapid diagnosis tests, microscopy, and polymerase chain reaction for detecting Plasmodium mixed infection: a systematic review and meta-analysis

July 30, 2020 - 14:10 -- Open Access
Author(s): 
Kotepui M, Kotepui KU, De Jesus Milanez G, Masangkay FR
Reference: 
Sci Rep. 2020 Jul 29; 10(1):12765

Malaria rapid diagnostic tests (RDTs) are widely used to detect malaria parasites among patients who suspected malaria infections in malaria-endemic areas where microscopy is unavailable. Nevertheless, little is known about the performance of RDTs in detecting Plasmodium mixed infections. The present study aimed to evaluate the discordant results between RDTs and microscopy/polymerase chain reaction (PCR) in detecting Plasmodium mixed infections.

One-step PCR: A novel protocol for determination of pfhrp2 deletion status in Plasmodium falciparum

July 27, 2020 - 12:21 -- Open Access
Author(s): 
Jones S, Subramaniam G, Plucinski MM, Patel D, Padilla J, Aidoo M, Talundzic E
Reference: 
PLoS ONE 15(7): e0236369

Histidine-rich protein 2 (HRP2) detecting rapid diagnostic tests (RDTs) have played an important role in enabling prompt malaria diagnosis in remote locations. However, emergence of pfhrp2 deleted parasites is threatening the efficacy of RDTs, and the World Health Organization (WHO) has highlighted surveillance of these deletions as a priority. Nested PCR is used to confirm pfhrp2 deletion but is costly and laborious.

Plasmodium falciparum histidine-rich protein 2 diversity in Ghana

July 20, 2020 - 15:10 -- Open Access
Author(s): 
Otchere Addai-Mensah, Bismarck Dinko, Alexander Yaw Debrahq, et al.
Reference: 
Malaria Journal 2020 19:256, 16 July 2020

In the absence of microscopy, Plasmodium falciparum histidine-rich proteins 2 (PfHRP2)-based rapid diagnostic tests (RDTs) are recommended for the diagnosis of falciparum malaria, particularly in endemic regions. However, genetic variability of the pfhrp2 gene threatens the usefulness of the test due to its impact on RDT sensitivity. This study aimed to investigate the diversity of pfhrp2 in malaria cases among children in Ghana.

Systematic review and meta-analysis of diagnostic accuracy of Loop-mediated isothermal amplification (LAMP) methods compared to microscopy, PCR, and rapid diagnostic tests, for malaria diagnosis

July 14, 2020 - 15:50 -- Open Access
Author(s): 
Picot S, Cucherat M, Bienvenu AL
Reference: 
Int J Infect Dis. 2020 Jul 10:S1201-9712(20)30551-8

Diagnosis is a challenging issue in the way to malaria elimination. LAMP could be an alternative to conventional methods. Then, it is of interest to evaluate the diagnostic accuracy of LAMP for malaria compared to microscopy, PCR, and RDTs.

Pages

Subscribe to RSS - RDTs