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diagnosis

Research: Assessment of competence of participants before and after 7-day intensive malaria microscopy training courses in Nigeria

June 9, 2015 - 11:36 -- Bart G.J. Knols
Author(s): 
Yetunde A. Olukosi, Chimere O. Agomo, Oluwagbemiga O. Aina, Samuel K. Akindele, Hilary I. Okoh, Margaret O. Akinyele, Olusola Ajibaye, Bassey A. Orok, Bamidele A. Iwalokun, Veronica Enya, Uche T. Igbasi, Samson Awolola
Reference: 
MWJ2015, 6, 6
 
Accuracy of malaria diagnosis by microscopy has been a challenge in Nigeria due to poor competence of microscopists and inability to report on malaria species other than Plasmodium falciparum. Short courses were conducted to improve the skills of laboratory personnel to perform malaria microscopy in public health facilities. Seven-day malaria microscopy courses were conducted annually between 2011 and 2013.The training courses contained theoretical and practical sessions. Impact of the training was evaluated by practical and theoretical pre- and post-training assessments on malaria slide reading, parasite enumeration and basic malariology. The 102 participants who completed the training consisted of medical laboratory scientists (62; 60.8%), medical laboratory technicians (24; 23.5%) and other healthcare workers (16; 15.7%). The knowledge of basic malariology (theory) at pre- and post-tests were 34% (95% CI 31.7-36.3%) and 74.9% (95% CI 71.8-78.0%), respectively (P<0.001). The mean slide reading detection, species and counting agreements in pre-training assessment were 48.9%, 27.9% and 0%, respectively, and in post-training 56.8%, 39.2% and 25%, respectively. The mean species agreements in picture test pre- and post-training were 21.9% and 55.1%, respectively. There were significant differences (P<0.05) in the median pre-test scores in picture tests and basic malariology of the three categories of participants but not in malaria slide reading and parasite counting tests. However, post-training, a significant difference in test scores of the three categories of participants was recorded only for basic malariology (P=0.0003). The 7-day malaria microscopy courses significantly increased the knowledge and microscopy skills of the trainees and were sufficient to bridge the significant difference in baseline microscopy skills of the different categories of trainees that participated in the training courses.

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Quantitative Detection of Plasmodium falciparum Using, LUNA-FL, A Fluorescent Cell Counter

September 10, 2020 - 14:51 -- Open Access
Author(s): 
Hashimoto M, Yokota K, Kajimoto K, Matsumoto M, Tatsumi A, Yamamoto K, Hyodo T, Matsushita K, Minakawa N, Mita T, Oka H, Kataoka M
Reference: 
Microorganisms. 2020 Sep 4;8(9):E1356

The microscopic examination of Giemsa-stained thin and/or thick blood films (Giemsa microscopy) is the standard method of malaria diagnosis. However, the results of the diagnosis significantly depend on the skills of clinical technicians. Furthermore, sample preparation and analysis are laborious and time-consuming. Therefore, in this study, we investigated if a commercially available fluorescent cell counter, LUNA-FL, was useful for the detection of Plasmodium parasite and the estimation of parasitemia.

NOT Open Access | Diagnosis of clinical malaria in endemic settings

August 11, 2020 - 07:36 -- NOT Open Access
Author(s): 
Varo R, Balanza N, Mayor A, Bassat Q
Reference: 
Expert Rev Anti Infect Ther. 2020 Aug 9

Malaria continues to be a major global health problem, with over 228 million cases and 405,000 deaths estimated to occur annually. Rapid and accurate diagnosis of malaria is essential to decrease the burden and impact of this disease, particularly in children. We aimed to review the main available techniques for the diagnosis of clinical malaria in endemic settings and explore possible future options to improve its rapid recognition.

Method for Malaria Diagnosis Based on Extractions of Samples Using Non-Invasive Techniques: An Opportunity for the Nursing Clinical Practice

August 5, 2020 - 16:17 -- Open Access
Author(s): 
Gómez-Luque A, Parejo JC, Clavijo-Chamorro MZ, López-Espuela F, Munyaruguru F, Belinchón Lorenzo S, Monroy I, Gómez-Nieto LC
Reference: 
Int J Environ Res Public Health. 2020 Jul 31;17(15):E5551

Malaria has been for millennia one of the best known and most destructive diseases affecting humans. Its high impact has aroused great interest for the development of new effective and reliable diagnostic techniques. Recently it has been recently published that hairs from mammal hosts are able to capture, hold and finally remove foreign DNA sequences of Leishmania parasites.

Diagnosis of Malaria Parasites Plasmodium spp. in Endemic Areas: Current Strategies for an Ancient Disease

December 23, 2019 - 14:25 -- Open Access
Author(s): 
Gitta B, Kilian N
Reference: 
Bioessays, 2019 Dec 12:e1900138

Fast and effective detection of the causative agent of malaria in humans, protozoan Plasmodium parasites, is of crucial importance for increasing the effectiveness of treatment and to control a devastating disease that affects millions of people living in endemic areas. The microscopic examination of Giemsa‐stained blood films still remains the gold‐standard in Plasmodium detection today.

Not Open Access | Asymptomatic malaria in the clinical and public health context

December 2, 2019 - 17:45 -- NOT Open Access
Author(s): 
Cheaveau J, Mogollon DC, Mohon MAN, Golassa L, Yewhalaw D, Pillai DR
Reference: 
Expert Rev Anti Infect Ther. 2019 Nov 21:1-14.

Historically, the global community has focused on the control of symptomatic malaria. However, interest in asymptomatic malaria has been growing, particularly in the context of malaria elimination.

Superbug: an emerging and quickly spreading threat to the race against malaria

February 17, 2017 - 09:46 -- Salomé Beaujouan

While the malaria death count in Cambodia dropped to just one case in 2016, a new threat to the race against the disease arises in south-eastern Asia: superbugs. A superbug is a drug-resistant, human-killing parasite that modern medicine struggles to combat.

Do you see patients with cerebral malaria? ...Or know someone who does?

March 21, 2014 - 11:16 -- Lakshmi Swamy

If you care for patients with cerebral malaria or know someone who does, then we would appreciate your help in distributing this short survey.
Please use the following link to complete the survey: Cerebral Malaria Diagnosis Survey

I am an ophthalmologist with a background in malaria biology, and I am leading a survey study to understand how clinicians diagnose cerebral malaria.

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