Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry appears to be a promising, rapid and reliable tool for the epidemiological surveillance of Anopheles vectors, including their identification and their infection status.
Malaria transmission is dependent on the propensity of Anopheles mosquitoes to bite humans (anthropophily) instead of other dead end hosts.
This study has shown that Anopheles is well known as the night-biting vector of malaria.
Malaria transmission in Colombia is highly variable in space and time. Using a species distribution model, we mapped potential distribution of five vector species including Anopheles albimanus, Anopheles calderoni, Anopheles darlingi, Anopheles neivai, and Anopheles nuneztovari in five Departments of Colombia where malaria transmission remains problematic.
Selection of oviposition sites by gravid females is a critical behavioral step in the reproductive cycle of Anopheles coluzzii, which is one of the principal Afrotropical malaria vector mosquitoes.
Plasmodium salivary sporozoites are the infectious form of the malaria parasite and are dormant inside salivary glands of Anopheles mosquitoes.
The four potential vector species all displayed apparent seasonality in relative abundance. While An. minimus s.l. was collected through the entire year, its abundance peaked in the season immediately after the wet season.
The results showed the in vitro killing efficacy of a protein produced by a mosquito strain of W. anomalus against malaria parasites.
Ace-1 gene duplication is evidently far more complex in An. gambiae and An. coluzzii than the better-studied mosquito Culex quinquefasciatus, which consequently can no longer be considered an appropriate model for prediction of phenotypic consequences. These require urgent further evaluation in Anopheles.
The AS-LAMP method could detect the ace-1 R mutation within 60 min, which is faster than conventional PCR-RFLP.