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LAMP

Performance of rapid diagnostic tests, microscopy, loop-mediated isothermal amplification (LAMP) and PCR for malaria diagnosis in Ethiopia: a systematic review and meta-analysis

October 9, 2021 - 09:56 -- Open Access
Author(s): 
Daniel Getacher Feleke, Yonas Alemu and Nebiyou Yemanebirhane
Reference: 
Malaria Journal 2021 20:384, 27 September 2021

Rapid accurate diagnosis followed by effective treatment is very important for malaria control. Light microscopy remains the “golden standard” method for malaria diagnosis. Diagnostic test method must have sufficient level of accuracy for detecting malaria parasites. Therefore, this study aimed to investigate the diagnostic accuracy of rapid diagnostic tests (RDTs), microscopy, loop-mediated isothermal amplification (LAMP) and/or polymerase chain reaction (PCR) for the malaria diagnosis in Ethiopia.

Loop-mediated isothermal amplification (LAMP) assays targeting 18S ribosomal RNA genes for identifying P. vivax and P. ovale species and mitochondrial DNA for detecting the genus Plasmodium

May 26, 2021 - 09:45 -- Open Access
Author(s): 
Chen X, Zhang J, Yang Z, et al.
Reference: 
Parasit Vectors. 2021 May 24;14(1):278

Loop-mediated isothermal amplification (LAMP) has been widely used to diagnose various infectious diseases. Malaria is a globally distributed infectious disease attributed to parasites in the genus Plasmodium. It is known that persons infected with Plasmodium vivax and P. ovale are prone to clinical relapse of symptomatic blood-stage infections. LAMP has not previously been specifically evaluated for its diagnostic performance in detecting P. ovale in an epidemiological study, and no commercial LAMP or rapid diagnostic test (RDT) kits are available for specifically diagnosing infections with P. ovale.

Multicopy targets for Plasmodium vivax and Plasmodium falciparum detection by colorimetric LAMP

May 26, 2021 - 09:11 -- Open Access
Author(s): 
Oscar Nolasco, Jhoel Montoya, Ana L. Rosales Rosas, Scarlett Barrientos, Anna Rosanas-Urgell and Dionicia Gamboa
Reference: 
Malaria Journal 2021 20:225, 19 May 2021

Loop-mediated isothermal amplification (LAMP) for malaria diagnosis at the point of care (POC) depends on the detection capacity of synthesized nucleic acids and the specificity of the amplification target. To improve malaria diagnosis, new colorimetric LAMP tests were developed using multicopy targets for Plasmodium vivax and Plasmodium falciparum detection.

Detection of malaria parasites in samples from returning US travelers using the Alethia Malaria Plus LAMP assay

April 14, 2021 - 16:33 -- Open Access
Author(s): 
Ljolje D, Abdallah R, Lucchi NW
Reference: 
BMC Res Notes. 2021 Apr 7;14(1):128

In this study, the performance of a commercially available malaria LAMP assay (Alethia® Malaria Plus LAMP) was evaluated using retrospective clinical samples obtained from travelers returning to the United States of America (USA). Recently, several laboratories in non-malaria endemic countries evaluated the use of the loop mediated isothermal amplification (LAMP) assays for the diagnosis of imported malaria cases. These tests are simpler than polymerase-chain reaction (PCR)-based assays and were shown to have high sensitivity. Much of malaria diagnoses in the USA, is undertaken at the state level using mainly microscopy and rapid diagnostic tests (RDTs). However, molecular tools offer greater sensitivity over microscopy and RDTs. A reliable, easy to perform molecular assay can provide a test of choice for the accurate detection of malaria parasites in places where expert microscopy is lacking and/or for the detection of low-parasite density infections.

Validation of SYBR green I based closed‐tube loop‐mediated isothermal amplification (LAMP) assay for diagnosis of knowlesi malaria

March 30, 2021 - 13:58 -- Open Access
Author(s): 
Meng Yee Lai, Choo Huck Ooi and Yee Ling Lau
Reference: 
Malaria Journal 2021 20:166, 25 March 2021

As an alternative to PCR methods, LAMP is increasingly being used in the field of molecular diagnostics. Under isothermal conditions at 65 °C, the entire procedure takes approximately 30 min to complete. In this study, we establish a sensitive and visualized LAMP method in a closed-tube system for the detection of Plasmodium knowlesi.

NOT Open Access | Malaria Screening Using Front-Line Loop-Mediated Isothermal Amplification: Fourteen-Month Experience in a Nonendemic Regional Hub-and-Spoke Laboratory Setting

December 8, 2020 - 11:06 -- NOT Open Access
Tags: 
Author(s): 
McGinnis E, Chan G, Hudoba M, Markin T, Yakimec J, Roland K
Reference: 
Am J Clin Pathol. 2020 Dec 7:aqaa173

We implemented front-line loop-mediated isothermal amplification (LAMP)-based malaria screening in our nonendemic multicenter health region to reduce reliance on microscopy without sacrificing diagnostic efficiency. We aimed to evaluate changes in test volumes, positivity rates, turnaround times, and approximate labor time savings resulting from implementation of LAMP-based malaria testing to assess the efficacy of the novel testing algorithm in our regional hub-and-spoke testing model.

Development of a Multiplex Loop-Mediated Isothermal Amplification (LAMP) Method for Simultaneous Detection of Spotted Fever Group Rickettsiae and Malaria Parasites by Dipstick DNA Chromatography

November 7, 2020 - 12:42 -- Open Access
Author(s): 
Moonga LC, Hayashida K, Kawai N, Nakao R, Sugimoto C, Namangala B, Yamagishi J
Reference: 
Diagnostics (Basel). 2020 Nov 2;10(11):E897

Spotted fever group (SFG) rickettsiae causes febrile illness in humans worldwide. Since SFG rickettsiosis's clinical presentation is nonspecific, it is frequently misdiagnosed as other febrile diseases, especially malaria, and complicates proper treatment. Aiming at rapid, simple, and simultaneous detection of SFG Rickettsia spp. and Plasmodium spp., we developed a novel multiple pathogen detection system by combining a loop-mediated isothermal amplification (LAMP) method and dipstick DNA chromatography technology.

Active case detection of malaria in pregnancy using loop-mediated amplification (LAMP): a pilot outcomes study in South West Ethiopia

September 1, 2020 - 09:45 -- Open Access
Author(s): 
Guluma Tadesse, Claire Kamaliddin, Cody Doolan, Ranmalee Amarasekara, Ruth Legese, Abu Naser Mohon, James Cheaveau, Delenasaw Yewhalaw and Dylan R. Pillai
Reference: 
Malaria Journal 2020 19:305, 27 August 2020

125 million women are pregnant each year in malaria endemic areas and are, therefore, at risk of Malaria in Pregnancy (MiP). MiP is the direct consequence of Plasmodium infection during pregnancy. The sequestration of Plasmodium falciparum parasites in the placenta adversely affects fetal development and impacts newborn birth weight. Importantly, women presenting with MiP commonly develop anaemia. In Ethiopia, the Ministry of Health recommends screening symptomatic women only at antenatal care visits with no formal intermittent preventive therapy. Since MiP can display low-level parasitaemia, current tests which include microscopy and RDT are challenged to detect these cases. Loop mediated isothermal Amplification (LAMP) technology is a highly sensitive technique for DNA detection and is field compatible. This study aims to evaluate the impact of active malaria case detection during pregnancy using LAMP technology in terms of birth outcomes.

Systematic review and meta-analysis of diagnostic accuracy of Loop-mediated isothermal amplification (LAMP) methods compared to microscopy, PCR, and rapid diagnostic tests, for malaria diagnosis

July 14, 2020 - 15:50 -- Open Access
Author(s): 
Picot S, Cucherat M, Bienvenu AL
Reference: 
Int J Infect Dis. 2020 Jul 10:S1201-9712(20)30551-8

Diagnosis is a challenging issue in the way to malaria elimination. LAMP could be an alternative to conventional methods. Then, it is of interest to evaluate the diagnostic accuracy of LAMP for malaria compared to microscopy, PCR, and RDTs.

NOT Open Access | Loop-mediated isothermal amplification (LAMP) assays for Anopheles funestus group and Anopheles gambiae complex species

March 17, 2020 - 15:14 -- NOT Open Access
Author(s): 
Kamber T, Koekemoer LL, Mathis A
Reference: 
Med Vet Entomol. 2020 Mar 10

Species of the genus Anopheles vary with regard to their vector capacity for Plasmodium spp., the causative agent of malaria, and their accurate identification is often required. Loop‐mediated isothermal amplification (LAMP) is a rapid, simple and low‐cost method for specific DNA amplification.

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