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PCR artesunate artemisia annua

Is PCR genotyping of Plasmodium falciparum a reliable tool for monitoring drug resistance?

February 9, 2013 - 19:15 -- Pierre Lutgen

A recent press release from Wellcome Trust Sanger Institute raised a lot of concern. Based on a scientific paper published in Nature, 13 June 2012, it appears that a single infected person could harbour many genetically different Plasmodium falciparum parasites. The team from Oxford University found that these parasite populations easily swap DNA to create new forms.

This evidently raises the question how far PCR (polymer chain reaction genotyping) can distinguish between recrudescence (or treatment failure) and re-infection by new bites from anopheles mosquitoes.

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