Persistence Pays: piggyBac Remobilization in Anopheles
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Dave O’brochta of the University of Maryland has long insisted that merely developing the capacity to perform germline transformation of mosquitoes is not enough to claim a full-blown forward-genetic manipulation system. However, once transposable elements and vectors were available for Aedes and Anopheles, the enthusiasm for developing fundamentally new information about germline transformation waned – at least for many who would use it. Not for all though.
Evidence suggests that this is a feasible goal using currently available interventions, augmented with newer tools such as vaccines, which are in development.
The inhibitory function of Dimilin (Diflubenzuron), mostly a chitin synthesis regulator, on the ecdysis of mosquitoes (Anopheles gambiae s.l., Culex quinquefasciatus) and housefly was evaluated in the field and in laboratory.
This system offers a flexible and safe approach to testing the in vivo activity of novel antimalarials.
The dependence of Plasmodium vivax on invasion mediated by Duffy binding protein (DBP) makes this protein a prime candidate for development of a vaccine.
As I write the title of this editorial I know that I don’t have the answer to it. But it is an issue that is a lot on my mind these days. Let me tell you why... 
To become widely diffused, best practices suggest that end-users should be included in product development to ensure that preferred attributes or traits are considered.
These observations clearly indicate AQ+SP combination therapy is hepatotoxic in experimental Swiss mice.