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anti-malarial resistance

Molecular surveillance of anti-malarial resistance Pfdhfr and Pfdhps polymorphisms in African and Southeast Asia Plasmodium falciparum imported parasites to Wuhan, China

November 28, 2020 - 16:22 -- Open Access
Tingting Jiang, Weijia Cheng, Yi Yao, Huabing Tan, Kai Wu and Jian Li
Malaria Journal 2020 19:434, 25 November 2020

Anti-malarial drug resistance is a severe challenge for eventual control and global elimination of malaria. Resistance to sulfadoxine-pyrimethamine (SP) increases as mutations accumulate in the Pfdhfr and Pfdhps genes. This study aimed to assess the polymorphisms and prevalence of mutation in these genes in the Plasmodium falciparum infecting migrant workers returning to Wuhan, China.

Mosquitoes as a feasible sentinel group for anti-malarial resistance surveillance by Next Generation Sequencing of Plasmodium falciparum

October 22, 2019 - 17:00 -- Open Access
Rebecca Smith-Aguasca, Himanshu Gupta, Estefania Uberegui, Mara Maquina, Francisco Saute, Krijn P. Paaijmans, Alfredo Mayor and Silvie Huijben
Malaria Journal 2019 18:351, 17 October 2019

Plasmodium falciparum drug resistance surveillance is key to successful disease control and eradication. Contemporary methods that only allow determination of prevalence of resistance are expensive, time consuming and require ethical considerations. A newer method involving Next Generation Sequencing (NGS) permits obtaining frequency of resistance while allowing to detect minority variants in mixed infections. Here, NGS was tested for P. falciparum resistance marker detection in mosquito samples as a feasible and suitable alternative for molecular resistance surveillance. Anopheles funestus were collected in southern Mozambique using CDC light traps and manual collections. DNA was extracted from either whole mosquito, head-thorax and abdomen separately or pools of five mosquitoes. These samples were screened for P. falciparum and if positive for k13, pfcrt, pfmdr1, pfdhps and pfdhfr mutations related to anti-malarial drug resistance with Sanger sequencing and NGS.

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Helminth-infected patients with malaria: a low profile transmission hub?

November 20, 2012 - 06:45 -- Open Access
Nacher M
Malaria Journal 2012, 11:376 (15 November 2012)

Eclipsed by the debates about malaria incidence and severity in individual patients, malaria transmission in helminth-infected persons has so far received very little attention.

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