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plasmodium berghei

NOT Open Access | RAGE modulatory effects on cytokines network and histopathological conditions in malarial mice

July 7, 2020 - 13:17 -- NOT Open Access
Chin VK, Chuah YK, Lee TY, Nordin N, Ibraheem ZO, Zakaria ZA, Hassan H, Basir R
Exp Parasitol. 2020 Jul 2:107946

This study was aimed at investigating the involvement of Receptor for Advanced Glycation End Products (RAGE) during malaria infection and the effects of modulating RAGE on the inflammatory cytokines release and histopathological conditions of affected organs in malarial animal model. Plasmodium berghei (P. berghei) ANKA-infected ICR mice were treated with mRAGE/pAb and rmRAGE/Fc Chimera drugs from day 1 to day 4 post infection. Survival and parasitaemia levels were monitored daily. At day 5 post infection, mice were sacrificed, blood were drawn for cytokines analysis and major organs including kidney, spleen, liver, brain and lungs were extracted for histopathological analysis. RAGE levels were increased systemically during malaria infection.

Defining multiplicity of vector uptake in transfected Plasmodium parasites

July 6, 2020 - 16:56 -- Open Access
Carrasquilla M, Adjalley S, Sanderson T, Marin-Menendez A, Coyle R, Montandon R, Rayner JC, Pance A, Lee MCS
Sci Rep. 2020 Jul 2;10(1):10894

The recurrent emergence of drug resistance in Plasmodium falciparum increases the urgency to genetically validate drug resistance mechanisms and identify new targets. Reverse genetics have facilitated genome-scale knockout screens in Plasmodium berghei and Toxoplasma gondii, in which pooled transfections of multiple vectors were critical to increasing scale and throughput.

Haem oxygenase protects against thrombocytopaenia and malaria-associated lung injury

July 2, 2020 - 13:56 -- Open Access
Isaclaudia G. de Azevedo-Quintanilha, Isabel M. Medeiros-de-Moraes, Hugo C. Castro-Faria-Neto, et al.
Malaria Journal 2020 19:234, 1 July 2020

Malaria-triggered lung injury can occur in both severe and non-severe cases. Platelets may interact with parasitized erythrocytes, leukocytes and endothelium. These interactions can lead to microvessel obstructions and induce release of inflammatory mediators. Induction of the haem oxygenase enzyme is important in the host’s response to free haem and to several other molecules generated by infectious or non-infectious diseases. In addition, an important role for the haem oxygenase-1 isotype has been demonstrated in experimental cerebral malaria and in clinical cases. Therefore, the present work aims to determine the influence of haem oxygenase in thrombocytopaenia and acute pulmonary injury during infection with Plasmodium berghei strain NK65.

A lipocalin mediates unidirectional heme biomineralization in malaria parasites

June 30, 2020 - 14:58 -- Open Access
Matz JM, Drepper B, Blackman MJ, et al.
Proc Natl Acad Sci U S A. 2020 Jun 29:202001153

During blood-stage development, malaria parasites are challenged with the detoxification of enormous amounts of heme released during the proteolytic catabolism of erythrocytic hemoglobin. They tackle this problem by sequestering heme into bioinert crystals known as hemozoin. The mechanisms underlying this biomineralization process remain enigmatic. Here, we demonstrate that both rodent and human malaria parasite species secrete and internalize a lipocalin-like protein, PV5, to control heme crystallization.

In silico and in vivo anti-malarial investigation on 1-(heteroaryl)-2-((5-nitroheteroaryl)methylene) hydrazine derivatives

June 30, 2020 - 14:23 -- Open Access
Azar Tahghighi, Seyed-Mahdi Mohamadi-Zarch, Hamzeh Rahimi, Mahya Marashiyan, Naseh Maleki-Ravasan and Ali Eslamifar
Malaria Journal 2020 19:231, 29 June 2020

Resistance of Plasmodium falciparum against common anti-malarial drugs emphasizes the need of alternative and more effective drugs. Synthetic derivatives of 1-(heteroaryl)-2-((5-nitroheteroaryl)methylene) hydrazine have showed in vitro anti-plasmodial activities. The present study aimed to evaluate the molecular binding and anti-plasmodial activity of synthetic compounds in vivo.

Antiplasmodial activity of Cocos nucifera leaves in Plasmodium berghei-infected mice

June 8, 2020 - 15:05 -- Open Access
Tayler NM, De Jesús R, Spadafora R, Coronado LM, Spadafora C
J Parasit Dis. 2020 Jun; 44(2):305-313

Plasmodium falciparum (P. falciparum) malaria presents serious public health problems worldwide. The parasite´s resistance to antimalarial drugs has proven to be a significant hurdle in the search for effective treatments against the disease. For this reason, the study of natural products to find new antimalarials remains a crucial step in the fight against malaria. In this study, we aimed to study the in vivo performance of the decoction of C. nucifera leaves in P. berghei-infected mice. We analyzed the effectiveness of different routes of administration and the acute toxicity of the extract.

NOT Open Access | Drug Repositioning: Antimalarial Activities of GABA Analogs in Mice Infected with Plasmodium berghei

June 8, 2020 - 14:49 -- NOT Open Access
Ayankunle A, Wakeel O, Kolawole O, Oyekale A, Ojurongbe O, Adeyeba O
Cent Nerv Syst Agents Med Chem. 2020 Jun 4

Drug repositioning is becoming popular due to the development of resistance to almost all the recommended antimalarials. Pregabalin and gabapentin are chemical analogs of gamma-aminobutyric acid (GABA) approved for the treatment of epilepsy and neuropathic pain.

NOT Open Access | von Willebrand factor increases in experimental cerebral malaria but is not essential for late-stage pathogenesis in mice

June 3, 2020 - 16:01 -- NOT Open Access
Kraisin S, Martinod K, Desender L, Pareyn I, Verhenne S, Deckmyn H, Vanhoorelbeke K, Van den Steen PE, De Meyer SF
J Thromb Haemost. 2020 Jun 2

Cerebral malaria (CM) is the most severe complication in malaria. Endothelial activation, cytokine release and vascular obstruction are essential hallmarks of CM. Clinical studies have suggested a link between von Willebrand factor (VWF) and malaria pathology.

Zygote morphogenesis but not the establishment of cell polarity in Plasmodium berghei is controlled by the small GTPase, RAB11A

June 1, 2020 - 16:01 -- Open Access
Patil H, Hughes KR, Lemgruber L, Philip N, Dickens N, Starnes GL, Waters AP
PLoS Pathog 16(5): e1008091

Plasmodium species are apicomplexan parasites whose zoites are polarized cells with a marked apical organisation where the organelles associated with host cell invasion and colonization reside. Plasmodium gametes mate in the mosquito midgut to form the spherical and presumed apolar zygote that morphs during the following 24 hours into a polarized, elongated and motile zoite form, the ookinete. Endocytosis-mediated protein transport is generally necessary for the establishment and maintenance of polarity in epithelial cells and neurons, and the small GTPase RAB11A is an important regulator of protein transport via recycling endosomes.

A Novel Chemically Differentiated Mouse Embryonic Stem Cell-Based Model to Study Liver Stages of Plasmodium berghei

May 25, 2020 - 07:51 -- Open Access
Tripathi J, Segeritz CP, Griffiths G, Bushell W, Vallier L, Skarnes WC, Mota MM, Billker O
Stem Cell Reports. 2020 May 20:S2213-6711(20)30150-8

Asymptomatic and obligatory liver stage (LS) infection of Plasmodium parasites presents an attractive target for antimalarial vaccine and drug development. Lack of robust cellular models to study LS infection has hindered the discovery and validation of host genes essential for intrahepatic parasite development.


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