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anopheles stephensi

Digital droplet PCR and IDAA for the detection of CRISPR indel edits in the malaria species Anopheles stephensi

February 17, 2020 - 14:35 -- Open Access
Author(s): 
Carballar-Lejarazú R, Kelsey A, Pham TB, Bennett EP, James AA
Reference: 
Biotechniques. 2020 Feb 10

CRISPR/Cas9 technology is a powerful tool for the design of gene-drive systems to control and/or modify mosquito vector populations; however, CRISPR/Cas9-mediated nonhomologous end joining mutations can have an important impact on generating alleles resistant to the drive and thus on drive efficiency. We demonstrate and compare the insertions or deletions (indels) detection capabilities of two techniques in the malaria vector mosquito Anopheles stephensi:

Experimental population modification of the malaria vector mosquito, Anopheles stephensi

December 24, 2019 - 17:26 -- Open Access
Author(s): 
Pham TB, Phong CH, Bennett JB, Hwang K, Jasinskiene N, Parker K, Stillinger D, Marshall JM, Carballar-Lejarazú R, James AA.
Reference: 
PLoS Genet 15(12): e1008440

Small laboratory cage trials of non-drive and gene-drive strains of the Asian malaria vector mosquito, Anopheles stephensi, were used to investigate release ratios and other strain properties for their impact on transgene spread during simulated population modification. We evaluated the effects of transgenes on survival, male contributions to next-generation populations, female reproductive success and the impact of accumulation of gene drive-resistant genomic target sites resulting from nonhomologous end-joining (NHEJ) mutagenesis during Cas9, guide RNA-mediated cleavage.

Gene silencing through RNAi and antisense Vivo-Morpholino increases the efficacy of pyrethroids on larvae of Anopheles stephensi

September 3, 2019 - 15:22 -- Open Access
Author(s): 
Agata Negri, Marco Ferrari, Riccardo Nodari, Edoardo Coppa, Valentina Mastrantonio, Sergio Zanzani, Daniele Porretta, Claudio Bandi, Sandra Urbanelli and Sara Epis
Reference: 
Malaria Journal 2019 18:294, 28 August 201

Insecticides are still at the core of insect pest and vector control programmes. Several lines of evidence indicate that ABC transporters are involved in detoxification processes against insecticides, including permethrin and other pyrethroids. In particular, the ABCG4 gene, a member of the G subfamily, has consistently been shown to be up-regulated in response to insecticide treatments in the mosquito malaria vector Anopheles stephensi (both adults and larvae).

Using sibship reconstructions to understand the relationship between larval habitat productivity and oviposition behaviour in Kenyan Anopheles arabiensis

August 27, 2019 - 22:06 -- Open Access
Author(s): 
Joel O. Odero, Ulrike Fillinger, Emily J. Rippon, Daniel K. Masiga and David Weetman
Reference: 
Malaria Journal 2019 18:286, 23 August 2019

Strategies for combatting residual malaria by targeting vectors outdoors are gaining importance as the limitations of primary indoor interventions are reached. Strategies to target ovipositing females or her offspring are broadly applicable because all mosquitoes require aquatic habitats for immature development irrespective of their biting or resting preferences. Oviposition site selection by gravid females is frequently studied by counting early instar larvae in habitats; an approach which is valid only if the number of larvae correlates with the number of females laying eggs. This hypothesis was tested against the alternative, that a higher abundance of larvae results from improved survival of a similar or fewer number of families.

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Genome annotation improvements from cross-phyla proteogenomics and time-of-day differences in malaria mosquito proteins using untargeted quantitative proteomics

August 9, 2019 - 15:17 -- Open Access
Author(s): 
Lisa Imrie, Thierry Le Bihan, Áine O'Toole, Paul V. Hickner, W. Augustine Dunn, Benjamin Weise, Samuel S. C. Rund
Reference: 
PLoS ONE 14(7): e0220225

The malaria mosquito, Anopheles stephensi, and other mosquitoes modulate their biology to match the time-of-day.

Status of insecticide resistance and its biochemical and molecular mechanisms in Anopheles stephensi (Diptera: Culicidae) from Afghanistan

July 29, 2019 - 15:52 -- Open Access
Author(s): 
Noor Halim Zahid Safi, Abdul Ali Ahmadi, Sami Nahzat, Supriya Warusavithana, Naimullah Safi, Reza Valadan, Atie Shemshadian, Marzieh Sharifi, Ahmadali Enayati and Janet Hemingway
Reference: 
Malaria Journal 2019 18:249, 26 July 2019

Insecticide resistance of Anopheles stephensi, the main malaria vector in eastern Afghanistan, has been reported previously. This study describes the biochemical and molecular mechanisms of resistance to facilitate effective vector control and insecticide resistance management.

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Anthropogenic Factors Driving Recent Range Expansion of the Malaria Vector Anopheles stephensi

March 20, 2019 - 16:12 -- Open Access
Author(s): 
Sinnathamby N. Surendran, Kokila Sivabalakrishnan, Ranjan Ramasamy, et al.
Reference: 
Frontiers in Public Health, 1 March 2019 Volume 7 Article 53

The malaria vector Anopheles stephensi is found in wide tracts of Asia and the Middle East.

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Influence of midgut microbiota in Anopheles stephensi on Plasmodium berghei infections

October 30, 2018 - 15:41 -- Open Access
Author(s): 
Devaiah Monnanda Kalappa, Pradeep Annamalai Subramani, Neena Valecha, et al.
Reference: 
Malaria Journal 2018 17:385, 25 October 2018

The use of microbial symbiont to reduce the competence of vectors involved in disease transmission has gained much importance in recent years as an emerging alternative approach towards disease control.

Speculation on the possibility for introducing Anopheles stephensi as a species complex: preliminary evidence based on odorant binding protein 1 intron I sequence

October 17, 2018 - 15:09 -- Open Access
Author(s): 
Samira Firooziyan, Navid Dinparast Djadid and Saber Gholizadeh
Reference: 
Malaria Journal 2018 17:366, 16 October 2018

Iran is going to eliminate malaria transmission from the country, precise species identification, especially in complex species will be helpful in the prevention of malaria resurgence in the country, mainly because of common fauna of Anopheles species and through border malaria and population movement within Afghanistan, Pakistan, and Iran.

Genotype and biotype of invasive Anopheles stephensi in Mannar Island of Sri Lanka

May 30, 2018 - 07:38 -- Open Access
Author(s): 
Sinnathamby N. Surendran, Kokila Sivabalakrishnan, Ranjan Ramasamy, et al.
Reference: 
Parasites & Vectors (2018) 11:3

The present study provides a more detailed molecular characterization of An. stephensiand suggests the presence of the type-form of the vector for the first time in Sri Lanka. The single mutation in the cox1 gene may be indicative of a founder effect causing the initial diversification of An. stephensi in Sri Lanka from the Indian form. 

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