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malaria parasites

Directing Traffic: Chaperone-mediated protein transport in malaria parasites

May 13, 2020 - 14:00 -- Open Access
Florentin A, Cobb DW, Kudyba HM, Muralidharan V
Cell Microbiol. 2020 May 9:e13215

The ability of eukaryotic parasites from the phylum Apicomplexa to cause devastating diseases is predicated upon their ability to maintain faithful and precise protein trafficking mechanisms. Their parasitic life cycle depends on the trafficking of effector proteins to the infected host cell, transport of proteins to several critical organelles required for survival, as well as transport of parasite and host proteins to the digestive organelles to generate the building blocks for parasite growth.

Field evaluation of DNA detection of human filarial and malaria parasites using mosquito excreta/feces

April 13, 2020 - 14:05 -- Open Access
Minetti C, Pilotte N, Zulch M, Canelas T, Tettevi EJ, Veriegh FBD, Osei-Atweneboana MY, Williams SA, Reimer LJ
PLoS Negl Trop Dis. 2020 Apr 8;14(4):e0008175

We recently developed a superhydrophobic cone-based method for the collection of mosquito excreta/feces (E/F) for the molecular xenomonitoring of vector-borne parasites showing higher throughput compared to the traditional approach. To test its field applicability, we used this platform to detect the presence of filarial and malaria parasites in two villages of Ghana and compared results to those for detection in mosquito carcasses and human blood.

Lapatinib, Nilotinib and Lomitapide Inhibit Haemozoin Formation in Malaria Parasites

April 6, 2020 - 15:29 -- Open Access
de Sousa ACC, Maepa K, Combrinck JM, Egan TJ
Molecules. 2020 Mar 29;25(7). pii: E1571

With the continued loss of antimalarials to resistance, drug repositioning may have a role in maximising efficiency and accelerating the discovery of new antimalarial drugs. Bayesian statistics was previously used as a tool to virtually screen USFDA approved drugs for predicted β-haematin (synthetic haemozoin) inhibition and in vitro antimalarial activity.

Conserved associations between G-quadruplex-forming DNA motifs and virulence gene families in malaria parasites

March 23, 2020 - 11:21 -- Open Access
Gage HL, Merrick CJ
BMC Genomics. 2020 Mar 17;21(1):236

The Plasmodium genus of malaria parasites encodes several families of antigen-encoding genes. These genes tend to be hyper-variable, highly recombinogenic and variantly expressed. The best-characterized family is the var genes, exclusively found in the Laveranian subgenus of malaria parasites infecting humans and great apes. Var genes encode major virulence factors involved in immune evasion and the maintenance of chronic infections. In the human parasite P. falciparum, var gene recombination and diversification appear to be promoted by G-quadruplex (G4) DNA motifs, which are strongly associated with var genes in P. falciparum. Here, we investigated how this association might have evolved across Plasmodium species – both Laverania and also more distantly related species which lack vars but encode other, more ancient variant gene families.

NOT Open Access | Functional homo- and heterodimeric actin capping proteins from the malaria parasite

March 10, 2020 - 16:23 -- NOT Open Access
Bendes ÁÁ, Chatterjee M, Götte B, Kursula P, Kursula I
Biochem Biophys Res Commun. 2020 Mar 2. pii: S0006-291X(20)30400-9

Actin capping proteins belong to the core set of proteins minimally required for actin-based motility and are present in virtually all eukaryotic cells. They bind to the fast-growing barbed end of an actin filament, preventing addition and loss of monomers, thus restricting growth to the slow-growing pointed end.

Virtual screening as a tool to discover new β-haematin inhibitors with activity against malaria parasites

March 2, 2020 - 15:39 -- Open Access
de Sousa ACC, Combrinck JM, Maepa K, Egan TJ
Sci Rep. 2020 Feb 25; 10(1):3374

Malaria remains a major public health problem. With the loss of antimalarials to resistance, the malaria burden will likely continue for decades. New antimalarial scaffolds are crucial to avoid cross-resistance. Here, we present the first structure based virtual screening using the β-haematin crystal as a target for new inhibitor scaffolds by applying a docking method. The ZINC15 database was searched for compounds with high binding affinity with the surface of the β-haematin crystal using the PyRx Virtual Screening Tool. Top-ranked compounds predicted to interact with β-haematin were submitted to a second screen applying in silico toxicity and drug-likeness predictions using Osiris DataWarrior.

An Endoplasmic Reticulum CREC Family Protein Regulates the Egress Proteolytic Cascade in Malaria Parasites

March 2, 2020 - 15:38 -- Open Access
Fierro MA, Asady B, Brooks CF, Cobb DW, Villegas A, Moreno SNJ, Muralidharan V
mBio. 2020 Feb 25;11(1). pii: e03078-19

The endoplasmic reticulum (ER) is thought to play an essential role during egress of malaria parasites because the ER is assumed to be required for biogenesis and secretion of egress-related organelles. However, no proteins localized to the parasite ER have been shown to play a role in egress of malaria parasites. In this study, we generated conditional mutants of the Plasmodium falciparum endoplasmic reticulum-resident calcium-binding protein (PfERC), a member of the CREC family. Knockdown of the PfERC gene showed that this gene is essential for asexual growth of P. falciparum.

A mevalonate bypass system facilitates elucidation of plastid biology in malaria parasites

February 17, 2020 - 14:50 -- Open Access
Swift RP, Rajaram K, Prigge ST, et al.
PLoS Pathog 16(2): e1008316

Malaria parasites rely on a plastid organelle for survival during the blood stages of infection. However, the entire organelle is dispensable as long as the isoprenoid precursor, isopentenyl pyrophosphate (IPP), is supplemented in the culture medium. We engineered parasites to produce isoprenoid precursors from a mevalonate-dependent pathway, creating a parasite line that replicates normally after the loss of the apicoplast organelle. We show that carbon-labeled mevalonate is specifically incorporated into isoprenoid products, opening new avenues for researching this essential class of metabolites in malaria parasites.

Exposure to Ebola Virus and Risk for Infection with Malaria Parasites, Rural Gabon

February 10, 2020 - 16:22 -- Open Access
Jessica L. Abbate, Pierre Becquart, Eric Leroy, Vanessa O. Ezenwa, Benjamin Roche
Emerging Infectious Diseases, Volume 26, Volume 26, Number 2—February 2020

An association between malaria and risk for death among patients with Ebola virus disease has suggested within-host interactions between Plasmodium falciparum parasites and Ebola virus. To determine whether such an interaction might also influence the probability of acquiring either infection, we used a large snapshot surveillance study from rural Gabon to test if past exposure to Ebola virus is associated with current infection with Plasmodium spp. during nonepidemic conditions.

NOT Open Access | Falcipain cysteine proteases of malaria parasites: An update

January 15, 2020 - 14:44 -- NOT Open Access
Rosenthal PJ
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Volume 1868, Issue 3

The malaria parasite Plasmodium falciparum expresses four related papain-family cysteine proteases known as falcipains. These proteases play critical roles in the parasite life cycle, and as such are potential targets for new modes of antimalarial chemotherapy, as discussed in this review.


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