Human malaria parasite species were originally acquired from other primate hosts and subsequently became endemic, then spread throughout large parts of the world.
Malaria remains one of the world's most important infectious diseases and is responsible for enormous mortality and morbidity.
Infection with Plasmodium knowlesi, a zoonotic primate malaria, is a growing human health problem in Southeast Asia.
To investigate comparative risk and consequences of knowlesi malaria during pregnancy, we reviewed (1) Sabah Health Department malaria-notification records created during 2012–2013, (2) prospectively collected data from all females with polymerase chain reaction (PCR)–confirmed malaria who were admitted to a Sabah tertiary care referral hospital during 2011–2014, and (3) malaria microscopy and clinical data recorded at a Sabah tertiary care women and children's hospital during 2010–2014.
This study demonstrates how gene expression in P. knowlesi blood-stage parasites can differ dramatically depending on whether the parasites are grown in vivo, with only one cycle of development ex vivo, or as an adapted isolate in long-term in vitro culture.
This study is the first to report the genetic diversity and natural selection of PkDBPαII of P. knowlesi from Borneo Island.
Multiplex PCR accelerate the speed in the diagnosis of malaria. The PlasmoNex™ PCR assay seems to
be more accurate than real-time PCR in the speciation of all five human malaria parasites.
In the present study, we have employed two-dimensional gel electrophoresis-coupled immunoblotting techniques and mass spectrometry to identify novel circulating markers in sera from P. knowlesi-infected patients.
Notifications of P. malariae/P. knowlesi in Sabah are increasing, with this trend likely reflecting a true increase in incidence of P. knowlesi and presenting a major threat to malaria control and elimination in Malaysia.
Urine and faeces were obtained from a Plasmodium knowlesi infected-Japanese macaque (Macaca fuscata) over the course of an experimentally induced infection. P. knowlesi DNA (PkDNA) extracted from urine and faeces were monitored by nested PCR targeting the P. knowlesi specific cytochrome b (cytb) gene.