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A novel population of memory-activated natural killer cells associated with low parasitaemia in Plasmodium falciparum-exposed sickle-cell trait children

April 10, 2020 - 17:07 -- Open Access
Loiseau C, Doumbo OK, Traore B, Brady JL, Proietti C, de Sousa KP, Crompton PD, Doolan DL
Clin Transl Immunology. 2020 Apr 2;9(4):e1125

The sickle‐cell trait phenotype is associated with protection from malaria. Multiple molecular mechanisms have been proposed to explain this protection, but the role of the host immune system has been poorly investigated. We hypothesised that cellular immunity to malaria may develop differently in sickle‐cell trait children (HbAS) and children with normal haemoglobin (HbAA) repeatedly exposed to Plasmodium falciparum (Pf).

Optimization of parasite DNA enrichment approaches to generate whole genome sequencing data for Plasmodium falciparum from low parasitaemia samples

March 31, 2020 - 16:04 -- Open Access
Zalak Shah, Matthew Adams, Kara A. Moser, Biraj Shrestha, Emily M. Stucke, Miriam K. Laufer, David Serre, Joana C. Silva and Shannon Takala-Harrison
Malaria Journal 2020 19:135, 30 March 2020

Owing to the large amount of host DNA in clinical samples, generation of high-quality Plasmodium falciparum whole genome sequencing (WGS) data requires enrichment for parasite DNA. Enrichment is often achieved by leukocyte depletion of infected blood prior to storage. However, leukocyte depletion is difficult in low-resource settings and limits analysis to prospectively-collected samples. As a result, approaches such as selective whole genome amplification (sWGA) are being used to enrich for parasite DNA. However, sWGA has had limited success in generating reliable sequencing data from low parasitaemia samples. In this study, enzymatic digestion with MspJI prior to sWGA and whole genome sequencing was evaluated to determine whether this approach improved genome coverage compared to sWGA alone. The potential of sWGA to cause amplification bias in polyclonal infections was also examined.

Correlation of malaria parasitaemia with peripheral blood monocyte to lymphocyte ratio as indicator of susceptibility to severe malaria in Ghanaian children

November 15, 2018 - 15:10 -- Open Access
Samuel Antwi-Baffour, Ransford Kyeremeh, Dorcas Buabeng, Jonathan Kofi Adjei, Claudia Aryeh, George Kpentey and Mahmood Abdulai Seidu
Malaria Journal 2018 17:419

The study comprised of 1629 (m = 896; f = 733) children up to 5 years presenting with clinical malaria as cases and 445 (m = 257; f = 188) apparently healthy children as controls. The results indicated that there was a significant positive correlation between the monocytes to lymphocytes ratio and the presence of parasites (p = 0.04) and the level of parasitaemia within the age group of 0–3 years (p = 0.02) and 4–5 years (p = 0.03).

Efficacy of OZ439 (artefenomel) against early Plasmodium falciparum blood-stage malaria infection in healthy volunteers

August 22, 2016 - 16:24 -- Open Access
James S. McCarthy, Mark Baker, Peter O'Rourke, Louise Marquart, Paul Griffin, Rob Hooft van Huijsduijnen and Jörg J. Möhrle
J. Antimicrob. Chemother. (2016) 71 (9): 2620-2627

These results confirm the antimalarial potential of artefenomel for use in a single-exposure combination therapy. The observations from this study support and will assist further clinical development of artefenomel.

Plasmodium falciparum parasitaemia and malaria among pregnant women at first clinic visit in the mount Cameroon Area

October 23, 2015 - 15:33 -- Open Access
Judith K. Anchang-Kimbi, Vera Ngenwie Nkweti, Helen Ngum Ntonifor, Tobias O. Apinjoh, Rolland Bantar Tata, Hanesh Fru Chi and Eric Akum Achidi
BMC Infectious Diseases 2015, 15:439

This study investigated the prevalence and risk factors for P. falciparum infection and malaria among pregnant women reporting for first antenatal care (ANC) clinic visit in the mount Cameroon area.

High-throughput tri-colour flow cytometry technique to assess Plasmodium falciparum parasitaemia in bioassays

October 30, 2014 - 16:50 -- Open Access
Regis W Tiendrebeogo, Bright Adu, Susheel K Singh, Daniel Dodoo, Morten H Dziegiel, Benjamin Mordmüller, Issa Nébié, Sodiomon B Sirima, Michael Christiansen, Michael Theisen
Malaria Journal 2014, 13:412 (20 October 2014)

Here, a high-throughput, three-parameter (tri-colour) flow cytometry technique based on mitotracker red dye, the nucleic acid dye coriphosphine O (CPO) and the leucocyte marker CD45 for enumerating live parasites in bioassays was developed.

Multilevel and geo-statistical modeling of malaria risk in children of Burkina Faso

August 11, 2014 - 17:57 -- Open Access
Samadoulougou S, Maheu-Giroux M, Kirakoya-Samadoulougou F, De Keukeleire M, Castro M, Robert A
Parasites & Vectors 2014, 7 :350 (29 July 2014)

The aims of this study were: i) to identify such individual, household, community, and climatic/environmental risk factors for malaria in children under five years of age, and ii) to produce a parasitaemia risk map of Burkina Faso.

Magnetic isolation of Plasmodium falciparum schizonts iRBCs to generate a high parasitaemia and synchronized in vitro culture

April 7, 2014 - 16:52 -- Open Access
Mata-Cantero L, Lafuente MJ, Sanz L, Rodriguez MS
Malaria Journal 2014, 13 :112 (21 March 2014)

The described procedure allows obtaining a large scale synchronized parasite culture at a high parasitaemia with less manipulations than other methods previously described.

Patient-, health worker-, and health facility-level determinants of correct malaria case management at publicly funded health facilities in Malawi: results from a nationally representative health facility survey

February 26, 2014 - 16:42 -- Open Access
Steinhardt LC, Chinkhumba J, Wolkon A, Luka M, Luhanga M, Sande J, Oyugi J, Ali D, Mathanga D, Skarbinski J
Malaria Journal 2014, 13 :64 (20 February 2014)

Among the 2,019 patients surveyed, 34% had confirmed malaria defined as presence of fever and parasitaemia on a reference blood smear. 


Not Open Access | Single nucleotide polymorphisms of ADRB2 gene and their association with susceptibility for Plasmodium falciparum malaria and asthma in an Indian population

October 16, 2013 - 18:41 -- NOT Open Access
Abdul Vahab Saadi, Himanshu Gupta, Kapaettu Satyamoorthy, et al.
Infection, Genetics and Evolution, Volume 20, December 2013, Pages 140-147

The study provides evidence for the proposed role of β2-adrenoreceptor mediated molecular mechanisms in etiology of malaria, as well as asthma disease and drug response, for further clinical and therapeutic application studies.


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