I want to share my research observations (on artemisinin resistance in Plasmodium falciparum) with malaria Professionals in this scientific community and welcome your candid comments.
I am a Ph.D student working on surveillance for artemisinin-resistant Plasmodium falciparum in dried blood spots sampled from Nigeria. I sequenced the K13 propeller domain at Macrogen using the PCR primers published in Nature Ariey 2014 titled 'A molecular marker of artemisinin resistant Plasmodium falciparum malaria'.
25 gDNA samples previously confirmed positive with regular PCR were selected for sequencing in the first phase. Surprisingly, the kelch protein was amplified in only one (1) sample which proved to be sensitive to artemisinin. The kelch protein was not amplified in the remaining 24 samples. A retrial experiment was run by Macrogen and it produced the same result.
I don’t have an explanation for this observation, please I need your candid comment on this. I will post some images of the sequence results(chromatogram).
I look forward to your response.