Reply to: Cod liver oil: a stronger prophylactic against malaria than vaccines!
Very good article. I will be going through some of these issues
Reply to: E-interview with Dr. Silas Majambere (Burundi, 1975)
My response to the reasons mentioned by Dr. Silas.
First of all I agree with both reasons. However, the major reason "NOT TO REACH ALL POTENTIAL BREEDING SITES" For effective LSM, it is extremely important to identify and mark the potential breeding sites in a defined area through Breeding Sites Assessment Survey (BSAS), otherwise the success of LSM will be compromise to a remarkable extent. If few left un-noticed, un-treated, the problem will be there. In Pakistan we have very good experience of BSAS and impact on disease incidence.
Reply to: Not Open Access | Sleeping on the floor decreases insecticide treated bed net use and increases risk of malaria in children under 5 years of age in Mbita District, Kenya
Quite revealing info, food for thought on how best we can align programs for vector control with empowerment of communities to improve their family income, and hence household spending, not towards treatment of malaria, but towards spending on "luxuries" such as beds for younger children, which profoundly have an impact on the risk of infection by malaria.
Reply to: Lessons from the successful national malaria campaign of Italy 1900-1962
Dear Dr Giancarlo,
You are correct. There was no miracle. (And Mussolini should not be given credit for any inspired thoughts on his part in the battle against malaria.) Italy in the 1920s was considered a centre for malaria research, and yet in 1924, the League of Nations Malaria Commission reported it still couldn't suggest 'any single plan for dealing with malaria which would certainly be permanently effective in actual practice'. But the Commission reported a year later, in 1925, after inspecting the antimalaria works in Palestine that others should follow the methods used there, and the people involved in Palestine with the antimalaria work were benefactors of the world (not just of the Palestine population). The result was that almost only Palestine/Israel (apart from North America and Europe) is shown as the only place in the world by 1970 as having eliminated malaria. The unique contribution made by Dr Kligler in 1922 in Palestine had been his emphasis on education. He stressed education was as important as destruction of the mosquito breeding sites (which breeding site destruction methods were different in every case according to the locality).
This emphasis on education is exportable for the benefit of malaria control in Africa, and Bill Jobin is correct to point out that several decades will be needed before real progress is achieved. But perhaps that is why the malaria professionals are too impatient in their search for the magic bullet, the quick fix, to realise or overlook the role that education plays in malaria elimination.
Reply to: Loop-Mediated Isothermal Amplification Method (LAMP): Low and Effective cost Novel Tool for Molecular Public Health
I have had trouble with negative control (no template)for a few month like others, at first was all OK with LAMP, but suddenly it wasn't work.
I have changed primer stock, betaine, dntp's, MgSO4, buffer, water (I use DNAse free). The mix is made in a different room to that I add the templated (with different pipet, too) I use filter tips.
To probe if I had primers contamination, I did a PCR with: just B3/F3, FIP/BIP and LB/FB primers and one mix with all primers (using Taq and its solutions, of course)with its negative control, respectively.. I realized all negative controls for each reaction were OK. Even the all primer mixed control was OK.
So I repeat the LAMP and again the same result the negative control is a lader pattern-like...will it be possible some LAMP reactive is contaminated?, but I change all a lot of time!..
And in this wall I have read that this problem seems to be comun.
Someone could fix it?
I'll be thankful if one of you could help me!